to set up the software for your use. This guide shows a workflow that uses application settings. BD FACSDiva Software Quick Reference Guide for the BD LSR II. Compensation Controls in the BD FACSDiva Software Reference Manual. • Default templates are now provided for certain instrument functions. If you are the first user of the day: Boot up the computer and log on to Windows. Wait 10 seconds, then Open the BD FACSDiva software and log on. Allow the.
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BD Biosciences welcomes customer input on corrections and suggestions for referencce. Your statistics view should look similar to the following: After recording a tube, a copy of the instrument settings in effect at the time of recording is stored with the tube. You must click Set for the new configuration to apply. Tip To avoid errors when subsetting populations, always keep the population hierarchy in view.
After you are done More information. BD Biosciences recommends that you determine an organization strategy before you generate a manua of data. All parameters listed in the Parameters tab are available for ratio calculations. Normal worksheets are titled SheetN by default, and they have gray-tinted tabs. No part of this guide may be reproduced or distributed.
No parts of this work may be reproduced in any form or by any means – graphic, electronic, or mechanical, including photocopying, recording, taping, or More information.
If an experiment is open and the current tube pointer is set, the window displays instrument settings for the current acquisition tube. This software is the property of Becton, Facsdica and Company.
Those without administrative access can view only their own experiments and any experiments that have been designated as shared. The Browser lists only experiments with that name, along with the currently open experiment. Locate and read topics specific to the operation you are performing. After you are done.
See Adding Users on page You can also use the Contents or Index tab to find information. Preview the printed report or set up the page for printing by choosing the corresponding selections nanual the File menu.
Spaces are not allowed. Using the Current Tube Pointer When an experiment is open, a gray pointer or plot icon appears next to tubes in the Browser. Complying with all applicable. The software defaults to version FCS 3.
BD FACSDiva Software Reference Manual
Whether viewed on one monitor or two, workspace windows can be resized and repositioned for the most efficient operator workflow. Higher voltages increase detector sensitivity, resulting in increased signal; lower voltages decrease detector sensitivity, resulting in decreased signal.
Locked templates and default templates provided rreference the software cannot be overwritten.
There might be a short delay as the software recalculates the plot scaling. Algorithms within BD FACSDiva software differentiate fluorescence signals from the Cytometer Setup and Tracking beads to calculate and report measurements detailing cytometer performance. The Account Administration dialog box appears showing only your user name, unless you referece administrative access.
Add a description to keywords in the Inspector window. A new field with a drop-down menu is displayed under the Institution field with the custom name and value that you entered. The starting value the lowest level is half the value entered.
BD FACSDiva Software Reference Manual |
New Cytometer Settings Adds new tube-specific cytometer settings as a child of the selected tube or new specimen-specific cytometer settings element as a child of the selected specimen. See Using the Tube Inspector on page Ratios are calculated from uncompensated linear data and are always reported in linear.
Deselect the checkbox to display only experiments containing the required information. Pages are numbered in the order in which they will be printed. A minimum of two objects must be selected for the button to be active. Comments can be viewed when you are importing an analysis template. This software may not be duplicated, reproduced, or copied in any form or by any means whatsoever, except as otherwise permitted by law. Use the Parameters tab to specify which parameter data should be sent and stored, to apply PMT amplification or electronic gain for FSCand to convert the parameter display to log.
BD FACSCanto II Quick Reference Manual
The computer is left on at all times. Click in the same header again to reverse the sort order. To activate acquisition controls, click the icon next to the tube you want to acquire.